IACUC Resources
Many institutions require accreditation from Association for Assessment and Accreditation of Laboratory Animal Care International, or AAALAC,
AAALAC is a private, nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs. https://www.aaalac.org/
Therefore, it is increasingly likely that your institutional IACUC will require a protocol when working with E. scolopes for each grant you receive.
Here are several key papers to consider when creating an IACUC protocol for E. scolopes
Abbo, L.A., Himebaugh, N.E., DeMelo, L.M., Hanlon, R.T. and Crook, R.J., 2021. Anesthetic Efficacy of Magnesium Chloride and Ethyl Alcohol in Temperate Octopus and Cuttlefish Species. Journal of the American Association for Laboratory Animal Science, 60(5), pp.556-567.
Download Paper
Butler-Struben HM, Brophy SM, Johnson NA, Crook RJ. In vivo recording of neural and behavioral correlates of anesthesia induction, reversal, and euthanasia in cephalopod molluscs. Frontiers in physiology. 2018 Feb 20;9:109.
Download Paper
Cooke GM, Anderson DB, Begout ML, Dennison N, Osorio D, Tonkins B, Kristiansen T, Fiorito G, Galligioni V, Ponte G, Andrews PL. Prospective severity classification of scientific procedures in cephalopods: Report of a COST FA1301 Working Group survey. Laboratory animals. 2019 Dec;53(6):541-63.
Download Paper
Crook RJ, Walters ET. Nociceptive behavior and physiology of molluscs: animal welfare implications. Ilar Journal. 2011 Jan 1;52(2):185-95.
Download Paper
Fiorito G, Affuso A, Basil J, Cole A, de Girolamo P, D’angelo L, Dickel L, Gestal C, Grasso F, Kuba M, Mark F. Guidelines for the Care and Welfare of Cephalopods in Research–A consensus based on an initiative by CephRes, FELASA and the Boyd Group. Laboratory animals. 2015 Oct;49(2_suppl):1-90.
Download Paper
Liguori GR, Jeronimus BF, de Aquinas Liguori TT, Moreira LF, Harmsen MC. Ethical Issues in the Use of Animal Models for Tissue Engineering: Reflections on Legal Aspects, Moral Theory, Three Rs Strategies, and Harm–Benefit Analysis. Tissue Engineering Part C: Methods. 2017 Dec 1;23(12):850-62.
Download Paper
Neff EP. Considering the cephalopod. Lab animal. 2019 Jan;48(1):19.
Download Paper
Winlow W, Polese G, Moghadam HF, Ahmed IA, Di Cosmo A. Sense and Insensibility–An Appraisal of the Effects of Clinical Anesthetics on Gastropod and Cephalopod Molluscs as a Step to Improved Welfare of Cephalopods. Frontiers in physiology. 2018;9.
Download Paper
